Cannabidiol in wound healing: validation of an experimental protocol

Resumen

Validating well-structured experimental protocols is essential to ensure reproducibility and data quality in preclinical studies. This work presents a rigorous methodological framework for assessing wound healing in a murine model using cannabidiol (CBD) ointments at 1.5% and 3% concentrations. A completely randomized block design (CRBD) was applied, using sex as a blocking factor. Thirty CD1 murin biomodels were considered and selected based on strict inclusion and exclusion criteria and housed individually under controlled conditions with environmental enrichment. The approval of the Bioethics Committee of the Agrarian University of Ecuador was proposed, with technical support from INSPI and CBD formulations provided by ALLYMA CORP. The protocol included an adaptation phase, standardized wound induction, daily treatment application, and 21-day clinical evaluation. Photographic documentation was standardized using a fixed metallic base. The results demonstrated successful execution across all phases, highlighting the structured statistical organization, control of external variables, and consistency in data collection. This preclinical study provides a valuable contribution to research involving cannabidiol and other underexplored compounds in alternative medicine, offering a solid, ethical, and reproducible methodological foundation for future investigations.